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:: Volume 6, Issue 2 (12-2019) ::
vacres 2019, 6(2): 37-41 Back to browse issues page
Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants
Mohammadali Malekan , Seyed Davar Siadat , Mohammadreza Aghasadeghi , Nader Shahrokhi , Sana Eybpoosh , Elnaz Afshari, Seyed fazlollah Mousavi *
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (271 Views)
Introduction: Streptococcus pneumoniae causes invasive and non-invasive diseases in children and adults. Currently, there are two types of pneumococcal vaccines: 23-valent pneumococcal polysaccharide vaccine and 13-valent pneumococcal conjugate vaccine which have caused many failures. Therefore, a new generation of pneumococcal vaccines is being pursued. Methods: An improved version of our previous study was performed using recombinant C-terminal of pneumococcal polyhistidine triad protein D (PhtD-C) as a vaccine antigen. The antigen was combined with meningococcal outer membrane vesicle (OMV) and alum as adjuvants to immunize BALB/c mice intraperitoneally. The generated total IgG, specific IgG, IgG1 and IgG2a antibodies and the killing ability of pneumococci by an opsonophagocytosis assay were then assayed. Results: Immunization by 30 µg PhtD-C and 50 µg OMV as an adjuvant, induced higher amounts of functional antibodies compared to our previous study while killing 50-55% of pneumococci cells. Conclusion: At optimized concentrations, PhtD-C and meningococcal OMV could be considered as a potent immunogens and the induced specific IgGs were effective and functional for killing pneumococci.
Keywords: Streptococcus pneumoniae|poly Histidine triad protein|PhtD-C|Opsonophagocytosis assay|outer membrane vesicle ,
Full-Text [PDF 527 kb]   (83 Downloads)    
Type of Study: Research | Subject: Vaccine development, efficacy and safety evaluation
Received: 2020/02/29 | Accepted: 2020/06/10 | Published: 2020/06/28
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Malekan M, Siadat S D, Aghasadeghi M, Shahrokhi N, Eybpoosh S, Afshari E et al . Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants. vacres. 2019; 6 (2) :37-41
URL: http://vacres.pasteur.ac.ir/article-1-175-en.html

Volume 6, Issue 2 (12-2019) Back to browse issues page
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