Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants

Malekan, Mohammadali; Siadat, Seyed Davar; Aghasadeghi, Mohammadreza; Shahrokhi, Nader; Eybpoosh, Sana; Afshari, Elnaz; Mousavi, Seyed fazlollah

doi:10.29252/vacres.6.2.37

دوره 6، شماره 2 - ( 9-1398 ) جلد 6 شماره 2 صفحات 41-37 | برگشت به فهرست نسخه ها

‎ 10.29252/vacres.6.2.37

Mendeley

Zotero

RefWorks

Malekan M, Siadat S D, Aghasadeghi M, Shahrokhi N, Eybpoosh S, Afshari E et al . Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants. vacres 2019; 6 (2) :37-41
URL: http://vacres.pasteur.ac.ir/article-1-175-fa.html

Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants. Vaccine Research. 1398; 6 (2) :37-41

URL: http://vacres.pasteur.ac.ir/article-1-175-fa.html

Assessment of PhtD C-Terminal Immunogenicity by Opsonophagocytosis Assay (OPA) with OMVs as Adjuvants

چکیده: (3235 مشاهده)

Introduction: Streptococcus pneumoniae causes invasive and non-invasive diseases in children and adults. Currently, there are two types of pneumococcal vaccines: 23-valent pneumococcal polysaccharide vaccine and 13-valent pneumococcal conjugate vaccine which have caused many failures. Therefore, a new generation of pneumococcal vaccines is being pursued. Methods: An improved version of our previous study was performed using recombinant C-terminal of pneumococcal polyhistidine triad protein D (PhtD-C) as a vaccine antigen. The antigen was combined with meningococcal outer membrane vesicle (OMV) and alum as adjuvants to immunize BALB/c mice intraperitoneally. The generated total IgG, specific IgG, IgG1 and IgG2a antibodies and the killing ability of pneumococci by an opsonophagocytosis assay were then assayed. Results: Immunization by 30 µg PhtD-C and 50 µg OMV as an adjuvant, induced higher amounts of functional antibodies compared to our previous study while killing 50-55% of pneumococci cells. Conclusion: At optimized concentrations, PhtD-C and meningococcal OMV could be considered as a potent immunogens and the induced specific IgGs were effective and functional for killing pneumococci.

متن کامل [PDF 527 kb] (1017 دریافت)

نوع مطالعه: پژوهشي | موضوع مقاله: Vaccine development, efficacy and safety evaluation
دریافت: 1398/12/10

بازنشر اطلاعات
	این مقاله تحت شرایط Creative Commons Attribution-NonCommercial 4.0 International License قابل بازنشر است.

کلیه حقوق این وب سایت متعلق به Vaccine Research می باشد.

طراحی و برنامه نویسی : یکتاوب افزار شرق

Designed & Developed by : Yektaweb

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.