Tue, Nov 4, 2025
Mina Mirzaee 
, Fatemeh Fotouhi , Hadiseh Shokohi , Parisa Roshani Asl , Hadi Bamehr , Golnaz Bahramali , Behrokh Farhmand * , Seyed Masoud Hosseini
, Fatemeh Fotouhi , Hadiseh Shokohi , Parisa Roshani Asl , Hadi Bamehr , Golnaz Bahramali , Behrokh Farhmand * , Seyed Masoud Hosseini
Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran
Abstract: (11 Views)
Introduction: Influenza, particularly Influenza A, remains a significant global health challenge due to its high morbidity and mortality rates. The hemagglutinin (HA) protein plays a critical role in viral entry, replication, and disease progression, while neuraminidase (NA) is essential for the forming and releasing of viral particles. Despite the ongoing challenge of HA protein variability, which leads to the emergence of new antigenic strains, this study focuses on enhancing antibody responses and achieving partial protection against influenza through the use of HA and NA proteins. Methods: A recombinant peptide vaccine targeting Influenza A was developed and tested in mice, showing strong immune responses and increased protection. Evaluated through computational, laboratory, and animal studies, the vaccine proved effective both alone and with an adjuvant. Prior bioinformatics analyses ensured its safety and stability, highlighting the promise of using immunodominant epitopes in recombinant vaccines for better influenza prevention. Results: The recombinant peptide vaccine against Influenza A viruses demonstrated strong immune responses and enhanced protection in a mice model. Evaluations under in silico, in vitro, and in vivo conditions confirmed the vaccine’s efficacy, both alone and with an adjuvant, through robust immune responses and cytokine production. These findings highlight the potential of bioinformatically guided immunodominant epitopes in recombinant vaccines for improved influenza control. Conclusion: This recombinant vaccine prototype shows strong immunogenicity and potential for effective influenza control in mice model.
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.