Volume 3, Issue 1 And 2 (5-2016)                   vacres 2016, 3(1 And 2): 21-25 | Back to browse issues page


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Mirzaei B, Moosavi S, Babaei R, Siadat S, Farjah A, Shahrooei M. Purification and evaluation of glycerol teichoic acid from biofilm forming Staphylococcus epidermidis as a putative vaccine candidate. vacres. 2016; 3 (1 and 2) :21-25
URL: http://vacres.pasteur.ac.ir/article-1-81-en.html
Microbiology Research Center (MRC) Pasteur Institute of Iran, Tehran, Iran, Department of Mycobacteriology and pulmonary research, Pasteur Institute of Iran, Tehran, Iran.
Abstract:   (5169 Views)

Introduction: Biofilm forming Staphylococcus epidermidis is a main causative agent of infections related to medical devices. Purification and evaluation of Gly-TA polysaccharide from a biofilm-forming S. epidermidis as a putative vaccine candidate were the main goals of the current study. Methods: Taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (Gly-TA) was purified from the above-mentioned strain and biochemical analyses including, Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1-NMR) were conducted for the recovered polysaccharide. Results: Following PCR confirmation of a S. epidermidis strain, Gly-TA was extracted and its biochemical compositions (i.e. N-acetyl glucose amine residues) were obtained. Conclusion: It is envisaged that Gly-TA polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by S. epidermidis.

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Type of Study: Short communication |
Received: 2016/09/21 | Accepted: 2016/09/21 | Published: 2016/09/21

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