Volume 3, Issue 1 And 2 (5-2016)                   vacres 2016, 3(1 And 2): 5-8 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Javadi F, Rahimi P, Modaressi M, Shafiee Ardestani M, Bolhassani A, Aghasadeghi M et al . Synthesis and characterization of physicochemical and immunological properties of recombinant NS3-G2 dendrimer conjugate. vacres 2016; 3 (1 and 2) :5-8
URL: http://vacres.pasteur.ac.ir/article-1-78-en.html
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran.
Abstract:   (6327 Views)

Introduction: An effective vaccine against HCV infection is not available. The non-structural protein 3 (NS3) of the virus as an important immunogenic candidate has been utilized in various modules. Nanostructured polymers have been recently used for efficient vaccine and drug delivery. The aim of the current study was the synthesis of rNS3-G2 conjugate and preliminary evaluation of its immunogenicity. Methods: The dendrimer was synthesized and conjugated with purified recombinant NS3 (rNS3) protein. The physicochemical properties of the conjugate were evaluated by Zeta potential, FT-IR spectra and confirmed by atomic force microscopy (AFM). Immunogenicity of the conjugate was assessed in BALB/c mice. Results: Synthesis and conjugation of dendrimer G2 with the protein were confirmed and immunological assays showed that the conjugated form of the antigen induced higher titer of IgG compared to rNS3 antigen alone. Conclusion: The results showed that the antigenic structure of rNS3 was maintained when conjugated with the biodegradable and biocompatible G2 dendrimers and the immunogenic properties of the antigen were enhanced. Therefore the new formulation may have potential as a vaccine candidate.

Full-Text [PDF 400 kb]   (2022 Downloads)    
Type of Study: Original article |
Received: 2016/08/14

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2022 All Rights Reserved | Vaccine Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.