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Volume 10, Issue 2 (12-2023)
vacres 2023, 10(2): 11-27 |
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Rezayatmand H, Farahzadeh G, Alipourfard I, Rezaeirad A, Alem M, Khazaei S, et al . Immunoinformatics Analyses of a Selection of Important Leishmania major Vaccine Epitopes: an in silico Approach. vacres 2023; 10 (2) :11-27
URL: http://vacres.pasteur.ac.ir/article-1-349-en.html
URL: http://vacres.pasteur.ac.ir/article-1-349-en.html
Hamed Rezayatmand 
, Golnoush Farahzadeh , Iraj Alipourfard , Arash Rezaeirad , Mahsa Alem , Sasan Khazaei , Davood Siamian , Negar Jahani , Faezeh Kalantarzadeh , Mohammad Arad Zandieh , Seyyed Amir Hosseini , Saeed Heydarian , Hamidreza Majidiani * , Azar Najafi
, Golnoush Farahzadeh , Iraj Alipourfard , Arash Rezaeirad , Mahsa Alem , Sasan Khazaei , Davood Siamian , Negar Jahani , Faezeh Kalantarzadeh , Mohammad Arad Zandieh , Seyyed Amir Hosseini , Saeed Heydarian , Hamidreza Majidiani * , Azar Najafi
Healthy Aging Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran
Abstract: (2366 Views)
Introduction: Cutaneous leishmaniasis (CL) due to Leishmania major (L. major) is a widespread vector-borne parasitic infection in subtropical areas. Numerous studies have been conducted to present possible vaccination candidates to address CL. In the present study, 18 L. major vaccine candidate antigens, namely gp46, CatL, CatB, grp78, H1, H2A, H2B, and H4, HSP60, HSP70, HSP83 (HSP90), HSP100, rP0, KMP11, STI-1, TSA, LeIF, and LACK were evaluated by in silico methods to find novel immunogenic epitopes. Methods: online predictions were performed regarding physicochemical, solubility, antigenicity, allergenicity, signal peptide, and transmembrane domains. Since four proteins (i.e., CatB, CatL, gp46, and grp78) were shown to possess signal peptides and transmembrane domains, they were further analyzed along with two antigenic proteins (STI-1 and H2A), regarding post-translational modifications (PTMs), structural (secondary and tertiary) predictions, and epitope mapping for B-cells, cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes against human leukocyte antigen (HLA) reference sets. Results: The world coverage of the CTL and HTL allele-epitope compositions were 96.34% and 41.78%, respectively. Finally, potentially-immunogenic CTL (n = 8) and antigenic HTL (n = 8) epitopes, which were strong IFN-γ inducers, along with 6 B-cell epitopes were selected. Conclusion: These epitopes are potential immunodominant regions among these antigens that upon further evaluations could be considered for a multi-epitope vaccine construction against CL
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.