Volume 8, Issue 2 (12-2021)                   vacres 2021, 8(2): 117-126 | Back to browse issues page

XML Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Yadegari Z, Razavi M R, Salehi M. Phylogenetic Analysis, Cloning and Expression of Delta Amastin Encoding Gene from Leishmania major as a Vaccine Candidate. vacres 2021; 8 (2) :117-126
URL: http://vacres.pasteur.ac.ir/article-1-297-en.html
Microbiology Research Center, Pasteur Institue of Iran, Tehran, Iran
Abstract:   (343 Views)
Introduction: Amastin is a surface glycoprotein in Leishmania species and one of the most important vaccine candidates due to its involvement in pathogenesis and being an essential virulence factor for parasite replication within the mammalian host cells. There are more than 60 copies of Amastin gene per genome of the parasite. Methods: Following phylogenetic analysis, a selected Amastin sequence was optimized and cloned with signal peptide in Escherichia coli. The recombinant protein was evaluated by SDG-PAGE and a specific antibody by Western blotting. Results: Among the Amastin sequences within different chromosomes of Leishmania major, the main type known as delta Amastin (primary distributed on chromosome 34) could be expressed in E. coli host and was confirmed by SDG-PAGE and Western blotting. Conclusion: Due to its copy number and evolutionary conservation and its role in pathogenesis, δ-Amastin is considered as an important vaccine candidate against leishmaniasis which could be expressed as a recombinant protein in E. coli.
Full-Text [PDF 906 kb]   (155 Downloads)    
Type of Study: Research | Subject: Prophylactic and therapeutic, and plant-based vaccines
Received: 2022/09/14

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 All Rights Reserved | Vaccine Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.