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:: Volume 1, Issue 1 (Vaccine Research 2014) ::
vacres 2014, 1(1): 28-30 Back to browse issues page
Extraction and Purification of Haemophilus influenzae Type b Lipooligosac‌charide by Modified Phenol Method
Amin Arsang, Shamsi Yari, Morteza Masoumi, Ali Nour Neamatollahi, Farzam Vaziri, Mehdi Nejati, Ahmad Reza Bahremand, Seyed Davar Siadat *
Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran, Department of Mycobacteriology & Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Abstract:   (18592 Views)

  Introduction : Haemophilus influenzae type b (Hib) is a Gram negative bacterium and one of the causative agents of acute bacterial meningitis, especially in infants and children less than 5 years old. Lipooligosaccharide (LOS), one of the virulence factors which plays an important role in pathogenesis of Hib, has multiple applications in diagnosis and conjugate vaccines. In this study, LOS extracted from two Hib standard strains (ATCC 39930 and ATCC 10214) were compared. Material and methods: LOS was extracted by a modified hot phenol method from the aqueous and phenol phase and its concentration and purity assayed . Protein c ontaminations of the samples were determined spectrophotometrically and their endotoxin contents were assayed by the Limulus amebocyte lysate (LAL). Result and Discussion: The yield of the extracted LOS from strain ATCC10214 was about 475 μg/ml and the protein contaminations of the samples were approximately 0.07 mg/ml, whereas strain ATCC39930 yielded 520 μg/ml of LOS with protein contamination of 0.08 mg/ml. The results showed that the production of LOS by both strains was similar and the variation observed was not statistically significant (p < 0.001). Vac Res , 2014, 1 (1): 29-31

Keywords: Haemophilus influenzae type b, Lipooligosaccharide, Meningitis
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Type of Study: Original article |
Received: 2014/02/16 | Accepted: 2014/05/27 | Published: 2014/07/13
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Arsang A, Yari S, Masoumi M, Nour Neamatollahi A, Vaziri F, Nejati M, et al . Extraction and Purification of Haemophilus influenzae Type b Lipooligosac‌charide by Modified Phenol Method. vacres. 2014; 1 (1) :28-30
URL: http://vacres.pasteur.ac.ir/article-1-28-en.html

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