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Volume 9, Issue 2 (12-2022)
vacres 2022, 9(2): 27-30 |
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Noori P, Sotoodehnejadnematalali F, Rahimi P, Siadat S D. Isolation of Extracellular Vesicles of Akkermansia muciniphila as a Potential Therapeutic Platform. vacres 2022; 9 (2) :27-30
URL: http://vacres.pasteur.ac.ir/article-1-267-en.html
URL: http://vacres.pasteur.ac.ir/article-1-267-en.html
Seyed Davar Siadat, PhD Department of mycobacteriology and pulmonary research, pasteur institute of Iran, Tehran, Iran.
Abstract: (824 Views)
Introduction: Extracellular vesicles (EVs), an active biological compounds have significant roles in pathogenesis and intercellular interactions in both Gram-negative and Gram-positive bacteria.. A. muciniphila represents 3–5% of the gut microbiota community and is well-known in individuals with healthy gut through mucin degradation. Moreover, its abundance inversely correlates with body weight. Recently, several studies have been addressed regarding to extracting and obtaining EVs and consequently, many methods are raised to isolate and characterize EVs. The goal of this study was to isolate A. muciniphila EVs from strain ATCC BAA-835 and found out the characterizing of their physico-chemical properties. Methods: The extraction of EVs from A. muciniphila strain ATCC BAA-835 was performed by ultracentrifuge technique. The size and shape of EVs were monitored by transmission electron microscope (TEM) and scanning electron microscope (SEM), and then EVs protein concentrations were assessed by Nano Drop and Bradford, Protein patterns of the EVs were evaluated by SDS-PAGE. Results: The extracted EVs were confirmed with the shape of vesicles and the sizes ranging from ~ 40 to 150 nm. Total protein concentration of EVs estimated 280nm and 595 nm from two methods; Nano Drop and Bradford, respectively.Conclusion: The results of the current study indicated that EVs shape, size and conformation were in accepted ranges. Although, subsequent analyses are necessary to clarify the safety, efficacy, practicality and mechanism of this bacterium EVs in clinical studies, especially as vaccine delivery vehicles in the case of vaccine researches.
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.