Showing 3 results for Lal
A Azizi Saraji , S Asiyabi , F Jalali , Mr Aghasadeghi, M Shahmehri , R Vahabpour ,
Volume 4, Issue 1 (5-2017)
Abstract
Introduction: Due to the role of neutralizing antibodies which can prevent human cytomegalovirus (HCMV) infection, most of the efforts have been focused on designing vaccines capable of eliciting protective humoral immunity. The aim of this study was to evaluate the antibody response of BALB/c mice to a truncated HCMV glycoprotein B produced in insect cells using Baculovirus Expression Vector System (BEVS). Methods: The ectodomain of HCMV gB coding sequence was synthesized and the recombinant protein was expressed in Spodoptera frugipedra (Sf9) insect cell line using BEVS. The expression of the recombinant HCMV gB was verified using an HRP-conjugated polyclonal antibody, specific for HCMV gB. The levels of antibody responses and characterization of the subclasses of IgG antibodies were evaluated after vaccination of the mice. Results: The expression of truncated HCMV gB protein (~ 70 kDa) in the infected insect cells was verified by Western blot analysis. Measurement of IgG subclasses showed the dominance of IgG1 subclass response among all of the IgG subclasses (P < 0.05) while the titers of IgG2a and IgG2b were approximately the same. Conclusion: This study demonstrated that BEVS could be used as an efficient approach for the expression of this truncated protein. The results also showed the use of this recombinant protein as a subunit vaccine could induce a significant antibody response, tilted toward IgG1.
Neena Verma, Sanjiva Bimal, Kumar Gupta Anil, Chandra Lal, Alok Ranjan, Rakesh Verma, K Pandey, Vidya Nand Rabidas, Shantanu Kar, P Das,
Volume 6, Issue 2 (12-2019)
Abstract
A B S T R A C T
Introduction: Control efforts of visceral leishmaniasis (VL) are hindered due to inappropriate early case detection of Leishmania infection with varying degree of susceptibility to develop the disease. Methods: We assessed the current infection status using Leishmanin skin test (LST) and direct agglutination test (DAT) in a cohort population (206 randomly selected individuals) in a VL endemic area of Bihar, India. Results: Cellular immunity was revealed in 18.4% and antibody response in 18.9% of the population. The age-group of 20-29 years were most vulnerable. DAT titer was inversely proportional to duration of past history of VL. The houses having present or past history of kala-azar in family were observed with high Leishmanin and DAT positivity, indicating relevance of household contacts in the disease transmission. Conclusion: The reactivity of both LST and DAT tests may help in identifying the possible groups with varying degree of susceptibility and risk of infection or having prior exposure to the leishmania infection with or without development of the disease.
Pegah Noori, Fattah Sotoodehnejadnematalali, Pooneh Rahimi, Seyed Davar Siadat,
Volume 9, Issue 2 (12-2022)
Abstract
Introduction: Extracellular vesicles (EVs), an active biological compounds have significant roles in pathogenesis and intercellular interactions in both Gram-negative and Gram-positive bacteria.. A. muciniphila represents 3–5% of the gut microbiota community and is well-known in individuals with healthy gut through mucin degradation. Moreover, its abundance inversely correlates with body weight. Recently, several studies have been addressed regarding to extracting and obtaining EVs and consequently, many methods are raised to isolate and characterize EVs. The goal of this study was to isolate A. muciniphila EVs from strain ATCC BAA-835 and found out the characterizing of their physico-chemical properties. Methods: The extraction of EVs from A. muciniphila strain ATCC BAA-835 was performed by ultracentrifuge technique. The size and shape of EVs were monitored by transmission electron microscope (TEM) and scanning electron microscope (SEM), and then EVs protein concentrations were assessed by Nano Drop and Bradford, Protein patterns of the EVs were evaluated by SDS-PAGE. Results: The extracted EVs were confirmed with the shape of vesicles and the sizes ranging from ~ 40 to 150 nm. Total protein concentration of EVs estimated 280nm and 595 nm from two methods; Nano Drop and Bradford, respectively.Conclusion: The results of the current study indicated that EVs shape, size and conformation were in accepted ranges. Although, subsequent analyses are necessary to clarify the safety, efficacy, practicality and mechanism of this bacterium EVs in clinical studies, especially as vaccine delivery vehicles in the case of vaccine researches.
