چکیده: (12 مشاهده)
Introduction: Most of the previous researches about Foot and Mouth Disease vaccine have stated that traditional and inactive vaccines cannot comprehensively control the disease and should be reconsidered. Currently, for this reason, the efforts of researchers are on the production and development of new generation vaccines. FMDV VP1 protein is one of the structural proteins and is crucial for the virus’s ability to infect host cells, elicitation of protective immune responses and the determination of serotype specificity. It plays a significant role in the virus’s antigenicity and is a target for vaccine development. Methods: The coding sequence of FMDV VP1 protein (O IR/P50/2016) was extracted from the NCBI site. Recombinant structure designed during heat shock method was transferred into Escherichia coli strain BL21. After culture of transformed bacteria and confirming the presence of the gene by PCR, restriction enzyme digestion and DNA sequencing, the recombinant FMDV VP1 protein was expressed and confirmed by SDS-PAGE and Western blotting. Results: The results of the electrophoresis, PCR and enzymatic digestion indicated that the FMDV VP1 protein gene was correctly inserted and cloned into pET-45 plasmid. SDS-PAGE and Western blotting demonstrated the existence of a protein with a molecular weight of 27 kDa. Conclusion: The results of the current study indicated that the prokaryotically expressed FMDV VP1 protein can potentially be considered as a replacement for old vaccines or antigens (in diagnostic kits) upon further investigations.
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