دوره 9، شماره 1 - ( 3-1401 )
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asiyabi S, Bamdad T, Pouriayevali M H, Chubin H. Establishment a Carcinoembryonic Antigen Stable Cell Line for Cancer Immunotherapy Study. vacres 2022; 9 (1) :1-7
URL: http://vacres.pasteur.ac.ir/article-1-292-fa.html
URL: http://vacres.pasteur.ac.ir/article-1-292-fa.html
Establishment a Carcinoembryonic Antigen Stable Cell Line for Cancer Immunotherapy Study. Vaccine Research. 1401; 9 (1) :1-7
چکیده: (1259 مشاهده)
Introduction: Cancer immunotherapy is one of the effective treatment methods that provide a better quality of life with limited side effects for patients. Carcinoembryonic Antigen (CEA) can be an appropriate target for cancer immunotherapy. Methods: A lentivector expressing CEA antigen, pCDH-CEA, was constructed by cloning CEA cDNA downstream of the CMV promoter. The constructed plasmid was co-transfected with helper plasmids, into Lenti-X 293 T cells. The lentivector-containing supernatant was collected. Titers of the CEA- lentivector were estimated using the RT-PCR method. The CT26 cells were then infected by CEA- lentivector. Puromycin as a selective antibiotic was added to the culture for 2 weeks to select CEA-positive cells. The ability to produce tumors in BALB/c mice was investigated. Results: The results showed that CEA expressing lentivector plasmids and the two other helper plasmids could be transfected into Lenti-X 293T cells efficiently and packaged successfully as a pseudo-lentivector. The detection of CEA mRNA and protein expression in the 6th and 14th passages of CT26-CEA cells was confirmed in the engineered stable cell line. Tumor formation was confirmed in cell inoculated mice. Conclusion: CT26-CEA cell line with stable expression of CEA can be used as a suitable tumor model to facilitate research on colorectal cancer in vitro and in mice models; therefore, it could be served as a valuable tool for cancer immunotherapy
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