Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.61186/vacres
en
jalali
1395
2
1
gregorian
2016
5
1
3
1
online
1
fulltext
fa
Purification and evaluation of glycerol teichoic acid from biofilm forming Staphylococcus epidermidis as a putative vaccine candidate
Short communication
Short communication
<p dir="ltr" style="text-align: justify;"><strong>Introduction:</strong> Biofilm forming <em>Staphylococcus epidermidis</em> is a main causative agent of infections related to medical devices. Purification and evaluation of Gly-TA polysaccharide from a biofilm-forming <em>S. epidermidis</em> as a putative vaccine candidate were the main goals of the current study. <strong>Methods:</strong> Taking advantage of size exclusion chromatography procedure, glycerol teichoic acid (Gly-TA) was purified from the above-mentioned strain and biochemical analyses including, Fourier Transform Infrared spectroscopy (FTIR) and Proton Nuclear Magnetic Resonance spectroscopy (H1-NMR) were conducted for the recovered polysaccharide. <strong>R</strong><strong>esults: </strong>Following PCR confirmation of a <em>S. epidermidis</em> strain, Gly-TA was extracted and its biochemical compositions (i.e. N-acetyl glucose amine residues) were obtained. <strong>Conclusion: </strong>It is envisaged that Gly-TA polysaccharide could be considered as a putative vaccine to inhibit formation of biofilm by <em>S. epidermidis</em>.</p>
Glycerol teichoic acid (Gly-TA), Staphylococcus epidermidis, FTIR, NMR.
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http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-1-23&slc_lang=fa&sid=1
B
Mirzaei
dr.bahman.m@gmail.com