en Other Other پژوهشي Research <span style="font-size:10pt"><span style="tab-stops:62.4pt"><span new="" roman="" style="font-family:" times=""><b><span style="letter-spacing:-.1pt">Introduction: </span></b><span style="letter-spacing:-.1pt">Influenza, particularly Influenza A, remains a significant global health challenge due to its high morbidity and mortality rates. The hemagglutinin (HA) protein plays a critical role in viral entry, replication, and disease progression, while neuraminidase (NA) is essential for the forming and releasing of viral particles. Despite the ongoing challenge of HA protein variability, which leads to the emergence of new antigenic strains, this study focuses on enhancing antibody responses and achieving partial protection against influenza through the use of HA and NA proteins. <b>Methods:</b> A recombinant peptide vaccine targeting Influenza A was developed and tested in mice, showing strong immune responses and increased protection. Evaluated through computational, laboratory, and animal studies, the vaccine proved effective both alone and with an adjuvant. Prior bioinformatics analyses ensured its safety and stability, highlighting the promise of using immunodominant epitopes in recombinant vaccines for better influenza prevention. <b>Results:</b> The recombinant peptide vaccine against Influenza A viruses demonstrated strong immune responses and enhanced protection in a mice model. Evaluations under <i>in silico</i>, <i>in vitro</i>, and <i>in vivo</i> conditions confirmed the vaccine&rsquo;s efficacy, both alone and with an adjuvant, through robust immune responses and cytokine production. These findings highlight the potential of bioinformatically guided immunodominant epitopes in recombinant vaccines for improved influenza control. <b>Conclusion:</b> This recombinant vaccine prototype shows strong immunogenicity and potential for effective influenza control in mice model.</span></span></span></span> Influenza virus, Multi-epitope, Peptide vaccine, Immunogenicity 49 55 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-8164-2&slc_lang=en&sid=1 Mina Mirzaee mirzaie.mina@ymail.com No Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, University of Shahid Beheshti, Tehran, Iran Fatemeh Fotouhi No Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran Hadiseh Shokohi No Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran Parisa Roshani Asl No Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran Hadi Bamehr No Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran Golnaz Bahramali No Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran. Behrokh Farhmand 0000-0002-7772-3595 Yes Department of Influenza and Respiratory Viruses, Pasteur Institute of Iran, Tehran, Iran Seyed Masoud Hosseini No Department of Microbiology and Microbial Biotechnology, Faculty of Life Science and Biotechnology, University of Shahid Beheshti, Tehran, Iran