en Reverse vaccinology Reverse vaccinology پژوهشي Research <span style="font-size:10pt"><span style="tab-stops:62.4pt"><span style="font-family:&quot;Times New Roman&quot;,serif"><b><span style="letter-spacing:-.1pt">Introduction: </span></b><span style="letter-spacing:-.1pt">Cutaneous leishmaniasis (CL) due to <i>Leishmania major</i> (<i>L. major</i>) is a widespread vector-borne parasitic infection in subtropical areas. Numerous studies have been conducted to present possible vaccination candidates to address CL. In the present study, 18 <i>L. major </i>vaccine candidate antigens, namely gp46, CatL, CatB, grp78, H1, H2A, H2B, and H4, HSP60, HSP70, HSP83 (HSP90), HSP100, rP0, KMP11, STI-1, TSA, LeIF, and LACK were evaluated by <i>in silico</i> methods to find novel immunogenic epitopes. <b>Methods: </b>online predictions were performed regarding physicochemical, solubility, antigenicity, allergenicity, signal peptide, and transmembrane domains. Since four proteins (<i>i.e.</i>, CatB, CatL, gp46, and grp78) were shown to possess signal peptides and transmembrane domains, they were further analyzed along with two antigenic proteins (STI-1 and H2A), regarding post-translational modifications (PTMs), structural (secondary and tertiary) predictions, and epitope mapping for B-cells, cytotoxic T-lymphocyte (CTL) and helper T-lymphocyte (HTL) epitopes against human leukocyte antigen (HLA) reference sets. <b>Results: </b>The world coverage of the CTL and HTL allele-epitope compositions were 96.34% and 41.78%, respectively. Finally, potentially-immunogenic CTL (n = 8) and antigenic HTL (n = 8) epitopes, which were strong IFN-&gamma; inducers, along with 6 B-cell epitopes were selected. <b>Conclusion: </b>These epitopes are potential immunodominant regions among these antigens that upon further evaluations could be considered for a multi-epitope vaccine construction against CL</span></span></span></span> Leishmania major, vaccine candidates, Immunoinformatics, Immunogenic epitopes 11 27 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-8007-1&slc_lang=en&sid=1 Hamed Rezayatmand h.rezayatmand.st@kmu.edu.ua No Department of Pharmacy and Technology of Organic Substances, Industrial Pharmacy, Ukrainian state, Chemical Technology University, Dnipro, Ukraine Golnoush Farahzadeh golif29@gmail.com No Pharmaceutical chemistry, faculty of chemistry, Azad Tehran north university, Tehran, IRAN Iraj Alipourfard ialipourfard@ichf.edu.pl No Institute of physical chemistry, Polish Academy of Science، Warsaw, Poland. Arash Rezaeirad arashrezaeirad@gmail.com No Institute of science, Molecular Biology, Üsküdar University, Istanbul, Türkiye Mahsa Alem m.alem@urmia.ac.ir No Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran. Sasan Khazaei sasan.kh91@gmail.com No Department of Parasitology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Davood Siamian Davood_Siyamiyan@gmail.com No Department of Biology, Faculty of Basic Science, Islamic Azad University, Tonekabon Branch, Mazandaran, Iran Negar Jahani negarjahani@gmail.com No Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran Faezeh Kalantarzadeh kalantarzadeh98@gmail.com No Faculty of Veterinary Medicine, Islamic Azad University, Shushtar Branch, Shushtar, Iran Mohammad Arad Zandieh M.aradzandieh@gmail.com No Faculty of Veterinary Medicine, Islamic Azad University, Science and Research Branch, Tehran, Iran Seyyed Amir Hosseini AmirHosseini718@yahoo.com No Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Saeed Heydarian s.heydarian@gmail.com No Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran Hamidreza Majidiani majidianih1@nums.ac.ir Yes Healthy Aging Research Centre, Neyshabur University of Medical Sciences, Neyshabur, Iran Azar Najafi azar.najafi98@gmail.com No Deputy of Research and Technology, Alborz University of Medical Sciences, Karaj, Iran