other Other Other پژوهشي Research <span style="font-size:10pt"><span style="tab-stops:310.55pt"><span new="" roman="" style="font-family:" times=""><b><span style="letter-spacing:-.1pt">Introduction: </span></b><span style="letter-spacing:-.1pt">The SARS-CoV-2 epidemic has infected and killed millions of people worldwide since 2019. Therapies available for SARS-CoV-2 disease are limited due to the sudden emergence of the virus. The need for appropriate vaccines to combat this dangerous virus is essential. Induction of specific antibodies against SARS-CoV-2 virus is mainly by nucleocapsid (N), membrane (M) and spike glycoprotein (S) proteins. On the other hand, cost-effective and fast methods for the expression and purification of recombinant proteins that retain antigenic properties are essential for vaccine development. <b>Methods:</b> A DNA fragment encoding the N, M and S1 proteins was inserted into pET28a vector to make an expression plasmid. The recombinant protein was then expressed in <i>Escherichia coli</i> Rosetta strain and purified by Ni-NTA column, followed by confirmation by SDS-PAGE and Western blotting. <b>Results:</b> PCR results showed that the gene was inserted correctly into the expression vector. The expression of the recombinant protein containing N, M and S1 proteins from SARS CoV-2 was optimized in a bacterial system. The recombinant protein was successfully purified from Ni-NTA column with a high yield <b>Conclusion:</b> Upon further evaluations, this cost-effective approach for the production of recombinant antigenic proteins in <i>E. coli</i> (Rosetta), could potentially be used for the development of vaccines against coronaviruses infections.&nbsp;</span></span></span></span> SARS-CoV-2, Recombinant, Protein, E. coli, pET28a, Vaccine. 6 10 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-2744-2&slc_lang=other&sid=1 Maryam Mashhadi Abolghasem Shirazi 0000-0001-6639-7221 No 1Department of Hepatitis and AIDS and Blood borne diseases, Pasteur Institute of Iran, Tehran, Iran. Mina Hannan 0000-0001-5721-1376 No Department of Hepatitis and AIDS and Blood borne diseases, Pasteur Institute of Iran, Tehran, Iran. Golnaz Bahramali 0000-0002-1762-4907 No Department of Hepatitis and AIDS and Blood borne diseases, Pasteur Institute of Iran, Tehran, Iran. Mohammad Reza Aghasadeghi 0000-0003-2186-0774 No Department of Hepatitis and AIDS and Blood borne diseases, Pasteur Institute of Iran, Tehran, Iran. Seyed Mehdi Sadat 0000-0001-7739-179X Yes Department of Hepatitis and AIDS and Blood borne diseases, Pasteur Institute of Iran, Tehran, Iran.