Vaccine Research

en Establishment a Carcinoembryonic Antigen Stable Cell Line for Cancer Immunotherapy Study Immunologic studies and animal models Immunologic studies and animal models Original article Original article Introduction: Cancer immunotherapy is one of the effective treatment methods that provide a better quality of life with limited side effects for patients. Carcinoembryonic Antigen (CEA) can be an appropriate target for cancer immunotherapy. Methods: A lentivector expressing CEA antigen, pCDH-CEA, was constructed by cloning CEA cDNA downstream of the CMV promoter. The constructed plasmid was co-transfected with helper plasmids, into Lenti-X 293 T cells. The lentivector-containing supernatant was collected. Titers of the CEA- lentivector were estimated using the RT-PCR method. The CT26 cells were then infected by CEA- lentivector. Puromycin as a selective antibiotic was added to the culture for 2 weeks to select CEA-positive cells. The ability to produce tumors in BALB/c mice was investigated. Results: The results showed that CEA expressing lentivector plasmids and the two other helper plasmids could be transfected into Lenti-X 293T cells efficiently and packaged successfully as a pseudo-lentivector. The detection of CEA mRNA and protein expression in the 6th and 14th passages of CT26-CEA cells was confirmed in the engineered stable cell line. Tumor formation was confirmed in cell inoculated mice. Conclusion:  CT26-CEA cell line with stable expression of CEA can be used as a suitable tumor model to facilitate research on colorectal cancer in vitro and in mice models; therefore, it could be served as a valuable tool for cancer immunotherapy Cancer immunotherapy, CEA, lentivector, RT-PCR, ELFA, 1 7 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-7417-1&slc_lang=en&sid=1 sanaz asiyabi sanazasiyabi@gmail.com 3181-0423-0002-0000 No Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Taravat Bamdad bamdad_t@modares.ac.ir 0701-3620-0003-0000 Yes Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran Mohammad hassan Pouriayevali m.h.pouria@gmail.com 1517-0133-0003-0000 No Department of Arboviruses and Viral Haemorrhagic Fevers (National Reference Laboratory), Pasteur institute of Iran, Tehran, Iran Hamzeh Chubin h.chubin13@gmail.com No Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran