Vaccine Research

other The Optimization of Faecalibacterium prausnitzii Extracellular Vesicle Concentrations on PPARγ and FIAF Expression in the Caco-2 Cell Culture Model Vaccine development, efficacy and safety evaluation Vaccine development, efficacy and safety evaluation Original article Original article Introduction: Faecalibacterium prausnitzii is an important member of human gut microbiota with a critical function in the health of humans through the induction of inflammatory and immune responses leading to intestinal hemostasis. Microbiota-induced extracellular vesicles (EVs) were presented as a novel communication pathway between microbiota and the host that are capable of imposing positive effects on the host. Recently, EV-based treatments have been evaluated in different studies, and using EVs derived from microbiota has been recommended in recent studies as post-vaccination adjuvants. Accordingly, in the present study, the effects of various EV concentrations on the mRNA expression of peroxisome proliferator-activated receptor γ (PPARγ) and fasting-induced adipose factor (FIAF) genes were evaluated and optimized in the human epithelial colorectal adenocarcinoma (Caco-2) cell line. Methods: The effects of the extracted EVs from F. prausnitzii on the PPARγ and FIAF gene expression were investigated in the Caco-2 cell line via cell culture and quantitative real-time PCR. The obtained outcomes were then compared with those of our previous study (concentrations of 50 and 100 µg/ml). Results: It was shown that F. prausnitzii EVs (150 µg/ml) significantly increased PPARγ and FIAF gene expression in the Caco-2 cell line, relative to previous studies conducted by our team. Conclusion: Considering the positive impact of F. Prausnitzii EVs on the studied genes’ expression in the present study, the EVs of the bacterium will be proposed as new post-vaccination adjuvants in people suffering from intestinal barrier disorders such as inflammatory bowel disease patients. However, more studies should be performed in this respect. F. prausnitzii, EVs, PPARγ, FIAF, Caco-2 cell line 39 43 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-6792-1&slc_lang=other&sid=1 Seyedeh Marzieh Moosavi 0000-0002-1836-1168 No Department of Microbiology, Faculty of Biology, Islamic Azad University, North Branch, Tehran, Iran. Abbas Akhavan Sepahi 0000-0002-4112-8962 No Department of Microbiology, Faculty of Biology, Islamic Azad University, North Branch, Tehran, Iran. Seyed Fazlollah Mousavi 0000-0003-0342-387X No Department of Microbiology, Pasteur Institute of Iran, Tehran, Iran. Farzam Vaziri 0000-0001-7788-2548 No Department of Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, Tehran, Iran. Seyed Davar Siadat 0000-0002-6892-5603 Yes Department of Mycobacteriology and Pulmonary Research Department, Pasteur Institute of Iran, Tehran, Iran. 4Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran