en Vaccine development, efficacy and safety evaluation Vaccine development, efficacy and safety evaluation Original article Original article <b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Introduction:</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"> Rabies is almost always fatal but entirely preventable through proper vaccination. Inadequacy of costly high-quality cell culture vaccines is sometimes a bottleneck for expanded rabies control plans. Reverse genetics along with other molecular biology means are trying to improve the immunogenicity and yield of rabies vaccine products. <b>Methods:</b> An additional glycoprotein gene of the rabies virus PV strain was inserted between the glycoprotein and polymerase genes of the virus. The viral proteins were expressed at the T7BHK cell line to rescue the recombinant virus. <b>Results:</b> The recombinant virus containing two consecutive glycoprotein genes was rescued from T7BHK cells. The virus particles were functional and successfully infected the permissive BSR cell line. <b>Conclusion:</b> The new virus strain with an additive copy of the glycoprotein gene has a good potential to be utilized in different studies, including cell biology and immunological properties of the rabies virus. In this study, the recombinant rabies virus was successfully rescued from cell culture which would pave the way for further investigations on this virus.</span></span></span> Rabies, Reverse Genetics, Rabies Glycoprotein, Virus Engineering 104 109 http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-131-10&slc_lang=en&sid=1 Ashkan Alamdary 0000-0001-7776-8809 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran Alireza Gholami algo2212@gmail.com 0000-0003-0706-2821 Yes Viral Vaccines Production Unit, Pasteur Institute of Iran Production and Research Complex, Karaj, Iran. Mohammad Azizi 0000-0003-1326-1755 No Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran. Zahra Noormohammadi 0000-0003-3890-9001 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.