Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.61186/vacres
en
jalali
1397
3
1
gregorian
2018
6
1
5
1
online
1
fulltext
fa
Cloning and expression of PMI1945 involved in iron acquisition as a promising vaccine candidate against Proteus mirabilis
Original article
Original article
<div style="text-align: justify;"><strong>Introduction:</strong> <em>Proteus mirabilis</em> is one of the common pathogens of urinary tract infections. Iron scavenger receptors from <em>P. mirabilis </em>are considered as important virulence factors of this strain and have the properties of an ideal vaccine candidate. In this study, the frequency of <em>P. mirabilis</em> iron receptor 1945 (PMI1945) was evaluated in the isolates and then its expression was conducted in pET28a-BL21. <strong>Methods:</strong> Amplification of <em>PMI1945 </em>was performed by PCR using <em>P. mirabilis </em>isolates genomic DNA. Cloning of <em>PMI1945 </em>gene was done in pET28a-BL21 system. After transformation, the expression of the cloned gene was induced by IPTG. The expression of this protein was then evaluated by SDS-PAGE and Western blot techniques. <strong>R</strong><strong>esults: </strong>The frequency of <em>PMI1945</em> gene in the isolates was 76%. The cloning of <em>PMI1945</em> gene into pET28a vector was confirmed by electrophoresis, PCR, enzyme digestion and sequencing. The sequencing of the cloned gene showed 100% identity with other sequences of <em>PMI1945</em> gene in GenBank. SDS-PAGE and Western blot results showed that 77 kDa PMI1945 protein was successfully expressed in BL21 (DE3) host. <strong>Conclusion: </strong>Cloning and expression of PMI1945 was done as the first step for evaluation of a novel vaccine candidate against UTIs caused by P. mirabilis.</div>
Proteus mirabilis, Iron scavenger receptors, PMI1945, Cloning, Expression.
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http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-1-53&slc_lang=fa&sid=1
B
Kavehei
kavehei.behnam@gmail.com