Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.61186/vacres
en
jalali
1397
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1
gregorian
2018
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online
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fulltext
fa
PorA typing of Neisseria meningitidis isolates from Iranian children for vaccine design
گزارش مورد
case report
<strong>Introduction:</strong> As the causative agent of meningitis, <em>Neisseria meningitidis</em> has different serogroups. The purpose of this study was to investigate the molecular properties of <em>N. meningitidis</em> strains among Iranian cases. <strong>Methods:</strong> 450 samples were collected from children under 5 years of age. Detection of <em>Neisseria</em> genus was done by phenotypic and genotypic methods. Multiplex PCR was used to identify the serogroups of N. meningitides. The sequencing of variable regions of <em>porA </em>gene was performed for detection of the subserogroups. <strong>Results:</strong> From 137 (30.44%) <em>Neisseria</em> isolates, 4 isolates (0.88%) belonged to <em>N. meningitidis</em> and 133 isolates (29.55%) belonged to other species. Multiplex PCR results showed that one isolate belonged to serogroup A while 3 belonged to serogroup B. The analysis of amplified VR1 and VR2 variable regions of porA showed 100% identity of the serogroup A strain with strain BZ83N and the serogroup B strains with strain 528 of <em>N. meningitidis</em> . In accordance with other findings in Asia, serogroups A and B were the most prevalent serogroups of <em>N. meningitidis</em>. Sequencing of variable regions of porA could identify the subserogroups of the isolates. <strong>Conclusion:</strong> sequencing of porA could be a valuable method for identification of <em>N. meningitidis</em> strains to be used in epidemiological studies as well as improved vaccine designs.
Neisseria meningitidis, porA, sequencing, PCR, typing.
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http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-1-51&slc_lang=fa&sid=1
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