Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.52547/vacres
en
jalali
1394
8
1
gregorian
2015
11
1
2
3
online
1
fulltext
en
Protein profiling and analysis of drug sensitive and multidrug resistant isolates of Mycobacterium tuberculosis by native polyacrylamide gel electrophoresis and mass spectrometry
Original article
Original article
<p dir="ltr" style="text-align: justify;"><strong>Introduction:</strong> Tuberculosis (TB) remains a deadly infectious disease despite all the efforts to reduce its incidence. Spread of multidrug resistant TB has seriously undermined the efforts to control the disease globally. In this study protein expression profile of MDR and sensitive isolates of MTB were analyzed and compared in order to identify proteins, which could be used in prevention, diagnosis and treatment. <strong>Methods:</strong> A sensitive and MDR isolate of <em>Mycobacterium tuberculosis</em> (MTB) were cultured on Middlebrook 7H9 medium and the whole cell lysates were subjected to native polyacrylamide gel electrophoresis (NPAGE) for protein expression profiling. Protein bands present in the MDR cell lysate that were not detected in the sensitive cell lysate were sent for identification by Matrix-assisted laser desorption/ionization time-of-flightmass spectrometry (MALDI-TOF-MS). <strong>R</strong><strong>esults: </strong>Comparison of the protein expression profiles showed 6 bands that were not detected in the sensitive isolates. MTB Structural Annotation database search of the mass spectrometry results identified these bands as Rv3597c, Rv0379,Rv3614c, Rv0475, Rv0462, andRv0147and global transcriptional regulation, involvement in cell wall and cell processes and intermediary metabolism and respiration were the functions attributed to these proteins. <strong>Conclusion: </strong>Our results highlighted the complexities of linking protein expression to MDR phenotype as none of the proteins identified could be linked directly to drug resistance. The proteins identified in the present study were mostly those essential for survival or virulence of the bacteria, and could be used for diagnosis or as candidate vaccine, but with a better understanding of the function of these proteins their association with the MTB resistance to antibiotics might become clear.</p>
MALDI-TOF-mass spectrometry, native PAGE, multidrug resistant, Mycobacterium tuberculosis.
81
85
http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-1-17&slc_lang=en&sid=1
Sh
Yari
shami14479@yahoo.com
10031947532846002065
10031947532846002065
Yes
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
AR
Hadizadeh Tasbiti
hadi@pasteur.ac.ir
10031947532846002066
10031947532846002066
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
M
Ghanei
10031947532846002067
10031947532846002067
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Chemical Injury Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
MA
Shokrgozar
10031947532846002068
10031947532846002068
No
National Cell Bank of Iran, Pasteur Institute of Iran, Tehran, Iran.
R
Mahdian
10031947532846002069
10031947532846002069
No
Molecular Medicine, Biotechnology Department, Pasteur Institute of Iran, Tehran, Iran.
A
Fateh
afateh2@gmail.com
10031947532846002070
10031947532846002070
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
SD
Siadat
d.siadat@gmail.com
10031947532846002071
10031947532846002071
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
F
Vaziri
farzam_vaziri@yahoo.com
10031947532846002072
10031947532846002072
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran, Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran.
Sh
Niknami
10031947532846002073
10031947532846002073
No
Department of Health Education, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
A
Bahrmand
padideh79@yahoo.com
10031947532846002074
10031947532846002074
No
Tuberculosis Department, Pasteur Institute of Iran, Tehran, Iran,