Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.52547/vacres
en
jalali
1401
3
1
gregorian
2022
6
1
9
1
online
1
fulltext
en
Phylogenetic Analysis, Cloning and Expression of Delta Amastin Encoding Gene from Leishmania major as a Vaccine Candidate
Prophylactic and therapeutic, and plant-based vaccines
Prophylactic and therapeutic, and plant-based vaccines
پژوهشي
Research
<span style="font-size:10pt"><span style="tab-stops:135.35pt 139.5pt 310.55pt"><span new="" roman="" style="font-family:" times=""><b><span style="letter-spacing:-.1pt">Introduction: </span></b><span style="letter-spacing:-.1pt">Amastin is a surface glycoprotein in <i>Leishmania</i> species and one of the most important vaccine candidates due to its involvement in pathogenesis and being an essential virulence factor for parasite replication within the mammalian host cells. There are more than 60 copies of Amastin gene per genome of the parasite. </span><b><span style="letter-spacing:-.1pt">Methods:</span></b><span style="letter-spacing:-.1pt"> Following phylogenetic analysis, a selected <i>Amastin</i> sequence was optimized and cloned with signal peptide in <i>Escherichia coli</i>. The recombinant protein was evaluated by SDG-PAGE and a specific antibody by Western blotting. </span><b><span style="letter-spacing:-.1pt">Results:</span></b><span style="letter-spacing:-.1pt"> Among the <i>Amastin</i> sequences within different chromosomes of <i>Leishmania major</i>, the main type known as delta Amastin (primary distributed on chromosome 34) could be expressed in <i>E. coli</i> host and was confirmed by SDG-PAGE and Western blotting. </span><b><span style="letter-spacing:-.1pt">Conclusion:</span></b><span style="letter-spacing:-.1pt"> Due to its copy number and evolutionary conservation and its role in pathogenesis, δ-Amastin is considered as an important vaccine candidate against leishmaniasis which could be expressed as a recombinant protein in <i>E. coli.</i></span></span></span></span>
Leishmania, Amastin, Recombinant, Vaccine
63
72
http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-7532-1&slc_lang=en&sid=1
Zahra
Yadegari
yadegari137@gmail.com
10031947532846004345
10031947532846004345
No
Department of biological science, Tehran north Branch, Islamic Azad University
Mohamad Reza
Razavi
mrrazavi@yahoo.com
10031947532846004346
10031947532846004346
Yes
Microbiology Research Center, Pasteur Institue of Iran, Tehran, Iran
Mitra
Salehi
Mitra_Salehi_Microbiology@yahoo.com
10031947532846004347
10031947532846004347
No
Department of biological science, Tehran north Branch, Islamic Azad University, Tehran, Iran