Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.52547/vacres
en
jalali
1400
3
1
gregorian
2021
6
1
8
1
online
1
fulltext
en
Construction and Rescue of a Rabies Virus with Duplicated Glycoprotein Gene
Vaccine development, efficacy and safety evaluation
Vaccine development, efficacy and safety evaluation
Original article
Original article
<b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt">Introduction:</span></span></span></b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="letter-spacing:-.1pt"> Rabies is almost always fatal but entirely preventable through proper vaccination. Inadequacy of costly high-quality cell culture vaccines is sometimes a bottleneck for expanded rabies control plans. Reverse genetics along with other molecular biology means are trying to improve the immunogenicity and yield of rabies vaccine products. <b>Methods:</b> An additional glycoprotein gene of the rabies virus PV strain was inserted between the glycoprotein and polymerase genes of the virus. The viral proteins were expressed at the T7BHK cell line to rescue the recombinant virus. <b>Results:</b> The recombinant virus containing two consecutive glycoprotein genes was rescued from T7BHK cells. The virus particles were functional and successfully infected the permissive BSR cell line. <b>Conclusion:</b> The new virus strain with an additive copy of the glycoprotein gene has a good potential to be utilized in different studies, including cell biology and immunological properties of the rabies virus. In this study, the recombinant rabies virus was successfully rescued from cell culture which would pave the way for further investigations on this virus.</span></span></span>
Rabies, Reverse Genetics, Rabies Glycoprotein, Virus Engineering
104
109
http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-131-10&slc_lang=en&sid=1
Ashkan
Alamdary
10031947532846003921
10031947532846003921
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Alireza
Gholami
algo2212@gmail.com
10031947532846003922
10031947532846003922
Yes
Viral Vaccines Production Unit, Pasteur Institute of Iran Production and Research Complex, Karaj, Iran.
Mohammad
Azizi
10031947532846003923
10031947532846003923
No
Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran.
Zahra
Noormohammadi
10031947532846003924
10031947532846003924
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.