Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.52547/vacres
en
jalali
1398
3
1
gregorian
2019
6
1
6
1
online
1
fulltext
fa
Design and Expression Optimization of a Chimeric Derivative of NetB, Alpha-Toxin and Metallopeptidase Proteins as a Subunit Vaccine Against Clostridium perfringens
Vaccine development, efficacy and safety evaluation
Vaccine development, efficacy and safety evaluation
پژوهشي
Research
Avian necrotic enteritis (NE) is a multi-virulence disease caused by the bacterium <em>Clostridium perfringens</em>. Several toxins of this bacteria are components of different candidate vaccines, and have been considered as important factors in pathogenesis of NE. A fusion subunit protein composed of immunodominant segments of NetB, Alpha-toxin and metallopeptidase proteins (NAM) was designed. The high level production of NE subunit vaccine candidate is important for the <em>in vitro</em> and <em>in vivo</em> evaluation and later to decrease the production costs. Therefore, the Response Surface Methodology (RSM) was used for optimizing <em>E. coli</em> culture condition. To this end, induction conditions including cell optical density prior induction (OD600nm), IPTG concentration, post-induction temperature and time were modified. The statistical analysis revealed that all variables except IPTG had significant effects on production and solubility of rNAM. A 7.39 fold increase in production of soluble rNAM was achieved when the post induction temperature, IPTG concentration, the pre-induction OD and time were 19 ºC, 0.55 mM, 0.8 respectively in 8 h after induction. Our study indicated that the RSM method is a simple and superior strategy for protein expression improvement which is considered as a major limitation in production of vaccine candidate and other recombinant proteins using different hosts.<br>
subunit vaccine, optimization, necrotic enteritis, toxin, RSM method
5
12
http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-125-2&slc_lang=fa&sid=1
Camellia
Katalani
Katalanik.bio@gmail.com
10031947532846002906
10031947532846002906
No
Agriculture Science and Natural Resource University (SANRU), Faculty of Agricultural science
Ghorbanali
Nematzadeh
gh.nematzadeh@sanru.ac.ir
10031947532846002907
10031947532846002907
Yes
Sari Agriculture Science and Natural Resource University (SANRU), Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT)
Gholamreza
Ahmadian
gholamrezaahmadian@yahoo.com
10031947532846002908
10031947532846002908
No
Department of Industrial and Environmental Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB)
Jafar
Amani
jafar.amani@gmail.com
10031947532846002909
10031947532846002909
No
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences5
Ghafar
Kiani
ghkiani@gmail.com
10031947532846002910
10031947532846002910
No
Agriculture Science and Natural Resource University (SANRU), Faculty of Agricultural science
Parastoo
Ehsani
p_ehsani@yahoo.com
10031947532846002911
10031947532846002911
No
Department of Molecular Biology, Pasteur Institute of Iran