Vaccine Research
Vaccine Research
vacres
Medical Sciences
http://vacres.pasteur.ac.ir
1
admin
2383-2819
2423-4923
10.52547/vacres
en
jalali
1397
9
1
gregorian
2018
12
1
5
2
online
1
fulltext
fa
A polyethylene glycol-based method for extraction of extracellular vesicles from Lactobacillus casei as vaccine delivery vehicle
Vaccine vectors, adjuvantsand immunomodulators
Vaccine vectors, adjuvantsand immunomodulators
Original article
Original article
<div style="text-align: justify;">Introduction The secretion of extracellular vesicles (EVs) has been neglected in Gram-positive bacteria due to the absence of an outer membrane and the difficulties of proper visualization. Here we aimed to prove that lactobacillus casei can secrete extracellular vesicles. Methods: EVs were extracted from Lactobacillus casei, cultured in De Man, Rogosa and Sharpe broth, using a polyethylene glycol (PEG) solution. The characteristics of the EVs were analyzed by electron microscopy, Dynamic Light Scattering (DLS) and SDS-PAGE. Results: The electron microscopy showed rounded vesicles with average diameter of 300 nm. The protein content of this nanostructure was 2.5 mg/ml with a protein pattern within the range of 10-200 kDa. DLS result showed populations of approximately 300 nm while the extracted EVs had a negative zeta potential. Conclusion: A new method of producing functional molecules from probiotic bacteria was presented. Our results indicated EVs purity with acceptable conformation. Further investigations are necessary to elucidate the efficacy, practicality and mechanism of action of such EVs in clinical practices, especially for development of bio-compounds and vaccine delivery vehicles.</div>
Lactobacillus casei, extracellular vesicles, polyethylene glycol.
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http://vacres.pasteur.ac.ir/browse.php?a_code=A-10-1-64&slc_lang=fa&sid=1
M
Ebrahimi Vargoorani
10031947532846002505
10031947532846002505
No
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
MH
Modarressi
10031947532846002506
10031947532846002506
No
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
E
Motevaseli
10031947532846002507
10031947532846002507
No
Department of Molecular Medicine, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.
F
Vaziri
10031947532846002508
10031947532846002508
No
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran.
SD
Siadat
d.siadat@gmail.com
10031947532846002509
10031947532846002509
Yes
Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran.