:: صفحه اصلي :: درباره نشريه :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 4، شماره 1 و 2 - ( 2-1396 ) ::
جلد 4 شماره 1 و 2 صفحات 41-45 برگشت به فهرست نسخه ها
Immunization with cytomegalovirus gB protein produced by the Baculovirus Expression Vector System to elicit humoral immune response in BALB/c mice
چکیده:   (832 مشاهده)
Introduction: Due to the role of neutralizing antibodies which can prevent human cytomegalovirus (HCMV) infection, most of the efforts have been focused on  designing vaccines capable of eliciting protective humoral immunity. The aim of this study was to evaluate the antibody response of BALB/c mice to a truncated HCMV glycoprotein B produced in insect cells using Baculovirus Expression Vector System (BEVS). Methods: The ectodomain of HCMV gB coding sequence was synthesized and the recombinant protein was expressed in Spodoptera frugipedra (Sf9) insect cell line using BEVS. The expression of the recombinant HCMV gB was verified using an HRP-conjugated polyclonal antibody, specific for HCMV gB. The levels of antibody responses and characterization of the subclasses of IgG antibodies were evaluated after vaccination of the mice. Results: The expression of truncated HCMV gB protein (~ 70 kDa) in the infected insect cells was verified by Western blot analysis. Measurement of IgG subclasses showed the dominance of IgG1 subclass response among all of the IgG subclasses (P < 0.05) while the titers of IgG2a and IgG2b were approximately the same. Conclusion: This study demonstrated that BEVS could be used as an efficient approach for the expression of this truncated protein. The results also showed the use of this recombinant protein as a subunit vaccine could induce a significant antibody response, tilted toward IgG1.
متن کامل [PDF 537 kb]   (248 دریافت)    
نوع مطالعه: Original article |
دریافت: ۱۳۹۶/۱۱/۱۱ | پذیرش: ۱۳۹۶/۱۱/۱۱ | انتشار: ۱۳۹۶/۱۱/۱۱
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA code



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Azizi Saraji A, Asiyabi S, Jalali F, Aghasadeghi M, Shahmehri M, Vahabpour R. Immunization with cytomegalovirus gB protein produced by the Baculovirus Expression Vector System to elicit humoral immune response in BALB/c mice. vacres. 2017; 4 (1 and 2) :41-45
URL: http://vacres.pasteur.ac.ir/article-1-107-fa.html

Immunization with cytomegalovirus gB protein produced by the Baculovirus Expression Vector System to elicit humoral immune response in BALB/c mice. تحقیقات واکسن. 1396; 4 (1 و 2) :41-45

URL: http://vacres.pasteur.ac.ir/article-1-107-fa.html



دوره 4، شماره 1 و 2 - ( 2-1396 ) برگشت به فهرست نسخه ها
تحقیقات واکسن Vaccine Research
Persian site map - English site map - Created in 0.22 seconds with 30 queries by YEKTAWEB 3735