:: صفحه اصلي :: درباره نشريه :: جستجو :: ثبت نام :: ارسال مقاله :: تماس با ما ::
:: دوره 4، شماره 1 و 2 - ( 2-1396 ) ::
جلد 4 شماره 1 و 2 صفحات 29-33 برگشت به فهرست نسخه ها
Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate
چکیده:   (1168 مشاهده)
Introduction: Human papillomavirus (HPV) 16 E7 protein is expressed constitutively by HPV-infected tumor cells. Mutant versions of E7 are considered as safer candidates for immunotherapy of cervical cancer.  Different strategies including formulation with adjuvants are used to induce a potent immune response against antigenic proteins. Methods: In this experimental study, we used Escherichia coli as a host to recombinantly express wild-type E7 and its mutant non-oncogenic form as E7GGG. We formulated both antigens with Montanide ISA 266 adjuvant and evaluated IFN-γ and IL-4 cytokines and antibody levels and also tumor regression in tumor-harboring C57BL/6 mice. Results: It was demonstrated that formulation of E7 and E7GGG antigens with Montanide ISA 266 resulted in a Th2-biased immune response. In the therapeutic mouse model, these formulations resulted in significant tumor regression compared to the control group. Conclusion: The formulation of the wild-type E7 and mutant E7GGG with Montanide ISA 266 might not be an optimal approach to regress TC-1 induced tumor; however, such combinations might be considered as an additive approach for stimulating the immune responses.
متن کامل [PDF 516 kb]   (376 دریافت)    
نوع مطالعه: Original article |
دریافت: ۱۳۹۶/۹/۲۱ | پذیرش: ۱۳۹۶/۹/۲۱ | انتشار: ۱۳۹۶/۹/۲۱
ارسال نظر درباره این مقاله
نام کاربری یا پست الکترونیک شما:

CAPTCHA code



XML   English Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mashhadi Abolghasem Shirazi M, Roohvand F, Arashkia A. Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate. vacres. 2017; 4 (1 and 2) :29-33
URL: http://vacres.pasteur.ac.ir/article-1-105-fa.html

Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate. تحقیقات واکسن. 1396; 4 (1 و 2) :29-33

URL: http://vacres.pasteur.ac.ir/article-1-105-fa.html



دوره 4، شماره 1 و 2 - ( 2-1396 ) برگشت به فهرست نسخه ها
تحقیقات واکسن Vaccine Research
Persian site map - English site map - Created in 0.18 seconds with 30 queries by YEKTAWEB 3749