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:: دوره 3، شماره 2 و 3 - ( 5-1395 ) ::
جلد 3 شماره 2 و 3 صفحات 64-68 برگشت به فهرست نسخه ها
Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate
چکیده:   (1059 مشاهده)
Introduction: Human papillomavirus (HPV) 16 E7 protein is expressed constitutively by HPV-infected tumor cells. Mutant versions of E7 are considered as safer candidates for immunotherapy of cervical cancer.  Different strategies including formulation with adjuvants are used to induce a potent immune response against antigenic proteins. Methods: In this experimental study, we used Escherichia coli as a host to recombinantly express wild-type E7 and its mutant non-oncogenic form as E7GGG. We formulated both antigens with Montanide ISA 266 adjuvant and evaluated IFN-γ and IL-4 cytokines and antibody levels and also tumor regression in tumor-harboring C57BL/6 mice. Results: It was demonstrated that formulation of E7 and E7GGG antigens with Montanide ISA 266 resulted in a Th2-biased immune response. In the therapeutic mouse model, these formulations resulted in significant tumor regression compared to the control group. Conclusion: The formulation of the wild-type E7 and mutant E7GGG with Montanide ISA 266 might not be an optimal approach to regress TC-1 induced tumor; however, such combinations might be considered as an additive approach for stimulating the immune responses.
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نوع مطالعه: Original article |
دریافت: ۱۳۹۶/۹/۲۱ | پذیرش: ۱۳۹۶/۹/۲۱ | انتشار: ۱۳۹۶/۹/۲۱
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Mashhadi Abolghasem Shirazi M, Roohvand F, Arashkia A. Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate. vacres. 2016; 3 (2 and 3) :64-68
URL: http://vacres.pasteur.ac.ir/article-1-105-fa.html

Preparation and in vivo anti-tumor evaluation of human papillomavirus E7 adjuvanted with Montanide ISA 266 as a vaccine candidate. تحقیقات واکسن. 1395; 3 (2 و 3) :64-68

URL: http://vacres.pasteur.ac.ir/article-1-105-fa.html



دوره 3، شماره 2 و 3 - ( 5-1395 ) برگشت به فهرست نسخه ها
تحقیقات واکسن Vaccine Research
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